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– Nitric oxide synthase (NOS) catalyzes the formation of nitric oxide (NO) from hypoxanthine-guanine phosphoribosyltransferase (HGPRT), the cofactor for this reaction is NADPH. It is widely known that this NADPH cofactor is not available in the normal living environment (at low oxygen tension) and that NOS cannot catalyze its conversion to NO from other free radicals (NO has a free electron and no donor). –

